Cloning and distribution of Ca2+-activated K+ channels in lobster Panulirus interruptus.
نویسندگان
چکیده
Large conductance Ca(2+)-activated potassium (BK) channels play important roles in controlling neuronal excitability. We cloned the PISlo gene encoding BK channels from the spiny lobster, Panulirus interruptus. This gene shows 81-98% sequence identity to Slo genes previously found in other organisms. We isolated a number of splice variants of the PISlo cDNA within Panulirus interruptus nervous tissue. Sequence analysis indicated that there are at least seven alternative splice sites in PISlo, each with multiple alternative segments. Using immunohistochemistry, we found that the PISlo proteins are distributed in the synaptic neuropil, axon and soma of stomatogastric ganglion (STG) neurons.
منابع مشابه
Informing Kelp Forest Restoration Site Selection with the California Spiny Lobster (panulirus Interruptus)
متن کامل
Behavioral responses to variable predation risk in the California spiny lobster Panulirus interruptus
Shelter dwelling and gregariousness are behavioral strategies used by benthic marine organisms to reduce the risk of predator-induced mortality. For spiny lobsters, the relative and synergistic success of shelter dwelling and aggregation in reducing mortality may vary with the perceived risk of predation as well as the availability and size distribution of conspecifics and shelters. We tested h...
متن کاملNeurodynamic control of the heart of freely moving spiny lobster (Panulirus japonicus)
The heart of the crustaceans has its own pacemaker neurons inside the heart, which are composed of 9 neurons. The neurons receive innervations of only three kinds of axons originated from the central nervous system; one pair of inhibitory and two pairs of acceleratory axons. Thus, in terms of the neural cardiac control from higher center, this system may have much more simplistic operation comp...
متن کاملNeurodynamic control of the heart of freely moving spiny lobster (Panulirus japonicus)
The heart of the crustaceans has its own pacemaker neurons inside the heart, which are composed of 9 neurons. The neurons receive innervations of only three kinds of axons originated from the central nervous system; one pair of inhibitory and two pairs of acceleratory axons. Thus, in terms of the neural cardiac control from higher center, this system may have much more simplistic operation comp...
متن کاملLobster shal: comparison with Drosophila shal and native potassium currents in identified neurons.
The transient potassium (K+) current, or A-current (IA), plays an essential role in shaping the firing properties of identified neurons in the 14-cell pyloric network in the stomatogastric ganglion of the spiny lobster, Panulirus interruptus. The different cells in the pyloric network have distinct IAs. To begin to understand the molecular basis for IA heterogeneity, we examined the relationshi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Neuroscience
دوره 170 3 شماره
صفحات -
تاریخ انتشار 2010